LGALS3 Knockout cell line (JIMT-1)

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LGALS3 Knockout cell line (JIMT-1) Order

LGALS3 Knockout cell line (JIMT-1)
Catalog#
YKO-H121
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LGALS3 Knockout cell line (JIMT-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LGALS3 Knockout cell line (JIMT-1)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
LGALS3
Gene ID
3958
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%DMEM+12%FBS
Accession
CVCL_E0WF(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript LGALS3-201)
Validation: PCR+Sanger Sequencing
LGALS3 Knockout cell line (JIMT-1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:JIMT-1		 STR Info (Cell bank)
Cell Line:JIMT-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 121314.0121314.0
D13S317 813813
D7S820 12121212
D16S539 11111111
VWA 18181818
TH01 7777
AMEL XXXX
TPOX 8888
CSF1PO 12131213
D12S391 1717
FGA 2123
D2S1338 2424
D21S11 2731.2
D18S51 1010
D8S1179 1013
D3S1358 1818
D6S1043 1111
PENTAE 719
D19S433 1213
PENTAD 912
D1S1656 1111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LGALS3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the JIMT-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LGALS3 gene knockout LGALS3 Knockout cell line (JIMT-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LGALS3 Knockout cell line (JIMT-1)
LGALS3 Knockout cell line (JIMT-1)
Catalog#
YKO-H121
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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